Abstract: A review on research progress in simultaneous speciation analysis of multi-element was presented,with emphasis on the applications of GC,LC,capillary electrophoresis hyphenated with spectrometry and mass spectrometry and the non-hyphenated methods such as electrochemical analysis and neutron activation analysis. The separation and enrichment methods for elemental species in the environment and biological samples were discussed briefly. The prospect of simultaneous speciation analysis of multi-element was also discussed (62 ref. cited).