基于铁氰化钐修饰的玻碳电极研究鸟嘌呤和腺嘌呤的电化学行为及其含量的测定

Study on the Electrochemical Behavior and Simultaneous Determination of Guanine and Adenine with the Samarium Ferricyanide Modified GCE

摘要: 利用电化学沉积法将铁氰化钐沉积在玻碳电极表面,形成铁氰化钐修饰电极,并利用循环伏安法研究了鸟嘌呤和腺嘌呤在该修饰电极上的电化学行为.相比裸玻碳电极,鸟嘌呤和腺嘌呤在修饰电极上的氧化峰电流增大,氧化峰电位降低,说明该修饰电极对鸟嘌呤和腺嘌呤的氧化具有良好的电催化能力.在优化条件下,两者的氧化峰电流与其浓度在5.0~70 μmol·L-1范围内呈现线性关系,检出限(3S/N)均为1.0 μmol·L-1.

Abstract: Samarium ferricyanide was deposited on the surface of GCE by electrochemical cyclic voltammetry (CV) to prepare the SmFe(CN)6 modified GCE.The electrocatalytic oxidation of guanine and adenine at the modified electrode was studied by CV.As compared with bare GCE,values of oxidation peak current of both guanine and adenine at the modified electrode were found to be increased while the oxidation peak potential to be decreased,showing that the modified electrode had good electrocatalytic activity toward the oxidation of guanine and adenine.Under optimum conditions,linear relationship was found between values of oxidation peak current and concentration of guanine and adenine in the same range of 5.0-70 μmol·L-1,with same detection limit (3S/N) of 1.0 μmol·L-1.