Abstract: Stainless steel wire was rapidly anodized and use as extraction fiber in SPME separation which was combined with the HPLC determination of benzoapyrene in water.It was found that the anodized stainless steel wire was easy to prepare,chemically and mechanically stable and durable.The water sample was extracted with the SPME fibrous head for 40 min at 30 ℃ with stirring at the rate of 1 000 r·min-1.The microextraction head was desorbed for 3 min.C18 reverse phase chromatographic column was used as stationary phase,and methanol-H2O (95+5) mixture was used as mobile phase in the chromatographic separation.UV-detection at 254 nm was adopted in the determination.Linear relationship between values of peak area and mass concentration of benzoapyrene was kept in the range of 0.1-100 μg·L-1,with detection limit (3S/N) of 10.92 ng·L-1.Tests for recovery were made by standard addition method at the concentration levels of 10 μg·L-1 and 50 μg·L-1,giving values of recovery in the ranges of 97.9%-110%.