Abstract: UHPLC was applied to the determination of residual domoic acid in shellfishes. The sample was extracted with methanol (1+1) solution. The extract was purified on Oasis MAX solid extraction column and the supernatant was separated on Waters ACQUITY UPLC BEH C18 chromatographic column with acetonitrile-0.1% (φ) trifluoroacetic acid solution (13+87) as the mobile phase for isocratic elution. UV-detection was made at the wavelength of 242 nm. The linearity range of domoic acid was 0.1-10.0 mg·L-1, with the lower limit of determination (10S/N) of 0.2 mg·kg-1. Recovery rates obtained by standard addition method were in the range of 76.8%-91.2%, and RSDs (n=6) were in the range of 11%-16%.