Abstract: A molecularly imprinted electrochemical sensor (MIECS) was prepared by electrochemical polymerization for recognition of bovine hemoglobin (BHb). Gold nanoparticles were electrodeposited on the surface of glassy carbon electrode, and then a layer of L-cysteine was self-assembled on the Au nanoparticles to functionize the surface of the modified electrode, and to introduce amino and carboxyl radicals to increase imprinted sites for recognition of protein. The molecularly imprinted polymer layer was synthesized by electrochemically induced redox polymerization of acrylamide using BHb as template, and the MIECS was formed after removing the template protein molecules by washing. The MIECS showed good response between the electrochemical signs and the mass concentration of BHb in the range of 1.0-1.0×103mg·L-1 with detection limit (3σ) of 0.5 mg·L-1. The proposed sensor exhibited to have high sensitivity, and could be used to identify the target protein.