离子色谱法测定葡萄酒中的山梨酸和苯甲酸的含量
Determination of Sorbic Acid and Benzoic Acid in Wine by Ion Chromatography
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摘要: 采用离子色谱法快速测定葡萄酒中的山梨酸和苯甲酸的含量。样品经超纯水稀释20倍,用聚酰胺粉末吸附提取溶液中的色素,净化液在IonPac AS11-HC阴离子色谱柱上分离,以30 mmol·L-1氢氧化钾溶液为淋洗液,采用抑制电导检测器检测,外标法定量。山梨酸和苯甲酸的质量浓度均在0.5~50 mg·L-1范围内与峰面积呈线性关系,方法的检出限(3S/N)在1.4~1.7 mg·L-1之间。加标回收率在97.7%~104%之间,测定值的相对标准偏差(n=6)在2.4%~3.4%之间。Abstract: Ion chromatography was applied to the rapid determination of sorbic acid and benzoic acid in wine. The sample was diluted 20 times with ultrapure water, and the pigment in the solution was adsorbed and extracted with polyamide powder. The purified solution was separated on an IonPac AS11-HC anion chromatographic column with 30 mmol·L-1 KOH solution as eluent. The analytes were detected by suppressed conductivity detector. External standard method was used for quantification. Linear relationship were found between the values of peak areas and the mass concentration of both sorbic acid and benzoic acid in the same range of 0.5-50 mg·L-1, with detection limits (3S/N) in the range of 1.4-1.7 mg·L-1. Values of recovery rates obtained by standard addition method were in the range of 97.7%-104% and RSDs (n=6) were in the range of 2.4%-3.4%.