Abstract: The brilliant ponceau molecularly imprinted polypyrrole material was prepared with pyrrole as functional monomers, brilliant ponceau as template molecular, ammonium persulfate as oxidant and sodium hydroxide solution as solvent to de-dope the template. The material was characterized by FT-IR. After optimization of the synthesis conditions, such as the concentration of brilliant ponceau solution, the volume of sulfuric acid and the polymerization time, the prepared molecularly imprinted material showed a good separation effect to selectively recognize isomers of brilliant ponceau and amaranth. The adsorption of brilliant ponceau on the molecularly inprinted polypyrrole material was in line with Freundlich mode, which was multi-molecular layer adsorption.