基于上转换发光纳米材料NaYF4: Yb,Er的免疫层析试纸条测定血清中的降钙素原

Determination of Procalcitonin in Serum with Immunochromatographic Strips Based on NaYF4: Yb,Er Upconverting Nanophosphors

摘要: 制备上转换发光纳米材料(UCP)——NaYF4: Yb,Er,进行表面功能化修饰后将其作为荧光标记物,制备了快速测定血清中降钙素原(PCT)的免疫层析试纸条。通过优化得到免疫层析反应的最佳条件: 抗体标记量为15 mg·g-1,UCP免疫复合物的用量为40 μg,检测线抗体划膜量为1.0 μL·cm-1,孵育时间为5 min。PCT的线性范围为0.05~50 μg·L-1,检出限(3s/k)为0.020 μg·L-1。应用制得的免疫层析试纸条测定血清样品中的PCT,结果与罗氏电化学发光法的结果一致,测定值的相对标准偏差(n=3)小于23%。

Abstract: Upconverting nanophosphors(UCP) NaYF4: Yb,Er were synthesized and surface functional modified. The prepared UCP were taken as fluorescent marker in the immunochromatographic strips for rapid determination of procalcitonin (PCT) in serum. The optimized conditions for immunochromatographic reaction were as follow: 15 mg·g-1 of labeled antibody, 40 μg of immune complexe (UCP), 1.0 μL·cm-1 of antibody zoned film on detection line and 5 min of incubation time. The linearity range of PCT was 0.05-50 μg·L-1 with the detection limit (3s/k) of 0.020 μg·L-1. The prepared immunochromatographic strips were used to determine PCT in serum, giving results in consistent with those obtained by Roche ECLIA and RSDs (n=3) less than 23%.