Abstract: QuEChERS-HPLC was applied to the simultaneous determination of 16 polycyclic aromatic hydrocarbons (PAHs) in rough rice and soil. The optimal conditions for QuEChERS were as follows: the sample was soaked overnight in acetonitrile, and extracted ultrasonically with the same solvent for 15 min, and then swirled for 2 min. After salting out and dehydration, the extract was cleaned up with PSA and C18E, and the purified extract was analyzed by HPLC -FLD and HPLC-DAD. Linearity for the 16 PAHs were kept between definite mass concentration ranges. Values of detection limits (3S/N) for the 16 PAHs were found in the range of 0.20-2.00 μg·L-1.Recovery was tested by addition of standards to the respective matrixes, values of recovery found were in the ranges of 85.0%-106% for brown rice, 81.5%-103% for rice hull and 76.7%-103% for soil, with RSDs (n=3) less than 7.5%.