Abstract: ICP-MS was applied to the determination of 17 metal elements, i.e., As, Pb and Sr, in Sea Cucumber. The sliced and torn sample of sea cucumber (0.500 g) was added with 8 mL of a mixture of nitric acid and hydrogen peroxide (6+20) and digested in a microwave oven under the selected program. The colorless and transparrent sample solution was used for the determination of 17 metal elements, using Ge, In, Bi and Sc as internal standards. Linear relationship between values of mass concentration of the 17 elements and the respecticve analytical signals were found in definite ranges, with detection limits (3s) in the range of 0.000 5-0.070 μg·L-1. Values of RSDs (n=6) ranged from 0.45% to 7.4%. The proposed method was used to determined the 17 elements in a CRM of GBW 100204, giving results in consistency with the certified values.