气相色谱-双柱双电子捕获检测器测定鱼肉中的有机氯农药

Determination of Organochlorine Pesticides in Fish by GC with Dual-Column and Dual-ECD

摘要: 采用气相色谱-双柱双电子捕获检测器（ECD）测定鱼肉样品中的10种有机氯农药（六氯苯、α-六六六、β-六六六、γ-六六六、δ-六六六、七氯、p，p'-DDE、o，p'-DDT、p，p'-DDD和p，p'-DDT）。样品经正己烷振荡提取后，提取液经自制酸性硅胶柱净化，采用DB-35MS和DB-5毛细管色谱柱进行双柱双ECD分析。10种有机氯农药的线性范围均为5.0~250 μg·L^-1，检出限（3.143s）在0.2~1.2 μg·kg^-1之间。加标回收率在85.7%~121%之间，测定值的相对标准偏差（n=6）不大于16%。

Abstract: GC with dual-column and dual-ECD was applied to the determination of 10 organochlorine pesticides in fish, including hexachlorobenzene, α-BHC, β-BHC, γ-BHC, δ-BHC, heptachlor, p,p'-DDE, o,p'-DDT, p,p'-DDD and p,p'-DDT. After the samples were extracted by oscillation with hexane, the extract was cleaned-up with self-made acidic silica gel column and the purified solution was analyzed by GC with dual-column (DB-35MS and DB-5) and dual-ECD system. The linearity ranges of 10 organochlorines were all 5.0-250 μg·L^-1, with detection limits (3.143s) in the range of 0.2-1.2 μg·kg^-1. Recovery rates obtained by standard addition method were in the range of 85.7%-121% and RSDs (n=6) were within 16%.