以金纳米粒子为共振光散射探针测定桑色素和杨梅苷含量

RLS Determination of Morin Hydrate and Myricetin Using Gold Nanoparticles as Probe

摘要: 利用种子生长法合成金纳米粒子（AuNPs），提出了以AuNPs为共振光散射（RLS）探针测定桑色素和杨梅苷含量的方法。通过静电引力，AuNPs可与桑色素或者杨梅苷结合，形成二元复合物，使RLS强度增强。桑色素/杨梅苷-AuNPs体系的RLS强度增加值ΔI_RLS与桑色素/杨梅苷的浓度呈线性关系，线性范围分别为0.40~10.0，0.10~10.0 μmol·L^-1，检出限（3S/N）分别为2.98，1.39 μg·L^-1。应用该方法测定了合成样品中的桑色素和杨梅苷的含量，加标回收率在97.2%~103%之间，测定值的相对标准偏差（n=5）在0.7%~2.5%之间。

Abstract: Gold nanoparticles (AuNPs) were synthesized by seed growth method, and resonance light scattering (RLS) was proposed for determination of morin hydrate and myricetin using AuNPs as probe. Morin hydrate or myricetin was bond to AuNPs by electrostatic interaction, which leaded to an obviously enhancement of RLS intensity. Linear relationships between ΔI_RLS and concentration were obtained in the range of 0.40-10.0 μmol·L^-1 for morin hydrate and 0.10-10.0 μmol·L^-1 for myricetin, with detection limits (3S/N) of 2.98, 1.39 μg·L^-1, respectively. The proposed method was used in the determination of morin hydrate and myricetin in synthesized samples. Test for recovery was made by standard addition method, giving values of recovery in the range of 97.2%-103%, with RSDs (n=5) ranged from 0.7% to 2.5%.