Abstract: The solid sample, e.g. cake, sausage and etc., (2.000 g) or liquid sample, e.g., drinks etc. (10.00 mL) was taken and diluted with some water. 2 mL of 220 g·L^-1 zinc acetate solution and 2 mL of 106 g·L^-1 potassium ferrocyanide solution were added to precipitate protein and to remove it by centrifuge. In case of semi-solid sample, e.g., jelly, 2.000 g were taken and diluted with water. The acidity was adjusted to pH 7 to 8 with ammonia (1+1) solution. The sample solution described above was made up to 25.0 mL with water, and separated by HPLC using TSK Eclipse XDB-C₁₈ column as stationary phase and mixed solution of methanol and triethylamin (1+9) (pH 5.0) as mobile phase. Linear relationships between values of peak area and mass concentration of benzoic acid and sorbic acid were found in the same range of 0.1-10.0 mg·L^-1, with detection limits (3S/N) of 1.8, 1.2 mg·kg^-1, respectively. Test for recovery was made by standard addition method at 3 concentration levels, giving results in the range of 90%-97%, with RSDs (n=6) less than 10%.