Abstract: Graphene and CuS nanoflower were prepared respectively by modified Hummers method and solvothermal method. And then nanoflower CuS and graphen were coated by dropping successively onto the surface of glassy carbon electrode, to prepare the nanoflower CuS-graphene modified GCE (abbrev. as nanoflower CuS/GF/GCE). The electrochemical behavior of vindesine at this modified electrode was studied by cyclic voltametry and different pulse voltametry. It was found that linear relationship between the magnitude of decrease of oxidation peak current and concentration of vindesine was obtained in the ranges of 1.0×10^-8 to 1.0×10^-7mol·L^-1, 1.0×10^-7 to 1.1×10^-5mol·L^-1 and 1.1×10^-5 to 1.0×10^-4mol·L^-1, with detection limit (3S/N) of 4.9×10^-9mol·L^-1. Precision of the method was tested at the concentration of 1.0×10^-6mol·L^-1 vindesine standard solution for 5 determinations, value of RSD found was 1.2%. The proposed method was applied to the analysis of drug samples of vindesine, giving values of recovery in the range of 97.1%-103%.