Abstract: High performance liquid chromatography (HPLC) method based on a fused-core sub-3 μm filler column was developed for the rapid determination of 25 drugs in analgesic health products. The samples were extracted ultrasonically with methanol, and the extract was separated on a Phenomenex Kinetex XB-C₁₈ chromatographic column (100 mm×4.6 mm, 2.6 μm) with acetonitrile/methanol (2+1) and 0.2 mol·L^-1 ammonium acetate solution (pH 4.2) as mobile phases for gradient elution. Diode array detector was used. Quantification analysis was performed by the external standard method. The same linearity range of 25 drugs was 1.0-100 mg·L^-1, with detection limits (3S/N) ranged from 0.02 g·kg^-1 to 0.10 g·kg^-1. Recovery rates obtained by standard addition method ranged from 90.6% to 110% with RSDs (n=6) of 0.42%-2.5%. The method was applied for analysis of 66 analgesic health products, and 24 positive samples were found and 8 drugs were detected.