高效液相色谱法测定纺织品中3种短链对特辛基苯酚乙氧基化物

HPLC Determination of 3 Kinds of Short Chain 4-(1,1,3,3-Tetramethylbutyl)phenol, Ethoxylated in Textiles

摘要: 样品经甲醇超声萃取2次，萃取液浓缩后用异丙醇定容，分析物在Dikama platisil silica硅胶柱（250 mm×4.6 mm，5.0 μm）上分离，以正己烷-异丙醇（85+15）混合液为流动相A、异丙醇-水（85+15）混合液为流动相B进行梯度洗脱。采用荧光检测器检测，激发波长为230 nm，发射波长为296 nm。3种对特辛基苯酚乙氧基化物的线性范围均为0.50~50 mg·L^-1，检出限（3S/N）在0.30~0.40 mg·kg^-1之间。加标回收率在94.4%~106%之间，测定值的相对标准偏差（n=6）小于6.0%。

Abstract: The sample was ultrasonically extracted twice with methanol. The extract was concentrated and then diluted with isopropanol. The analytes were seperated on a Dikama Platisil silica column (250 mm×4.6 mm, 5.0 μm), with N-hexane-isopropanol (85+15) as mobile phase A, isopropanol-water (85+15) as mobile phase B for gradient elution. Fluoresence detector was used with the excitation wavelength of 230 nm and the emission wavelength of 296 nm. Linearity ranges of the 3 kinds of 4-(1,1,3,3-tetramethylbutyl)phenol, ethoxylated were all 0.50-50 mg·L^-1, with detection limits (3S/N) of 0.30-0.40 mg·kg^-1. Recovery rates obtained by standard addition method were in the range of 94.4%-106% and RSDs (n=6) were less than 6.0%.