磁性固相萃取-高效液相色谱法测定葡萄酒中罗丹明B的含量

Magnetic Solid Phase Extraction-HPLC Determination of Rhodamine B in Wine

摘要: 样品溶液（pH 4.0，10 mL）以流量1.0 mL·min^-1经自制磁性石墨烯（CoFe₂O₄-G）20 mg萃取，用1 mL丙酮洗脱。所得溶液中的罗丹明B采用高效液相色谱法进行测定。罗丹明B的质量浓度在0.50~10 μg·L^-1内与其峰面积呈线性关系，检出限（3S/N）为0.08 μg·L^-1。按标准加入法进行回收试验，回收率为99.0%~101%，相对标准偏差（n=11）为1.6%~4.0%。

Abstract: The sample solution (pH 4.0, 10 mL) was extracted with homemade magnetic graphene (CoFe₂O₄-G) of 20 mg at the flow rate of 1.0 mL·min^-1, and acetone of 1 mL was used as the eluent. Rhodamine B in the above solution was determined by HPLC. Linear relationship between peak area and mass concentration of rhodamine B was found in the range of 0.50-10 μg·L^-1, with detection limit (3S/N) of 0.08 μg·L^-1. Test for recovery was made by standard addition method, giving results in the range of 99.0%-101%, with RSDs (n=11) ranged from 1.6% to 4.0%.