胶体金免疫层析法检测水产品中15种喹诺酮类药物

Detection of 15 Quinolones in Aquatic Products by Colloidal Gold Immunochromatography Assay

摘要: 采用胶体金免疫层析法检测水产品中恶喹酸、恩诺沙星、环丙沙星、二氟沙星、氟甲喹、沙拉沙星、达氟沙星、诺氟沙星、培氟沙星、依诺沙星、那氟沙星、氧氟沙星、奥比沙星、洛美沙星和麻保沙星等15种喹诺酮类药物。水产品样品用0.1%（体积分数）甲酸乙腈溶液提取，正己烷脱脂，胶体金试纸条检测，检测时间只需3~5 min。15种喹诺酮类药物的检出限在0.3~5 μg·kg^-1之间。对400批次样品进行检测，发现9批次阳性样品，经超高效液相色谱-串联质谱法（UHPLC-MS/MS）验证，检测结果一致。

Abstract: Colloidal gold immunochromatography assay was applied to the detection of 15 quinolones, i.e. Oxolinic acid, Enrofloxacin, Ciprofloxacin, Difloxacin, Flumequine, Sarafloxacin, Danofloxacin, Norfloxacin, Pefloxacin, Enoxacin, Nadifloxacin, Ofloxacin, Orbifloxacin, Lomefloxacin and Marbofloxacin in aquatic products. The sample of aquatic product was extracted with 0.1%(φ) formic acid acetonitrile solution, and n-hexane was added for defatting. Finally the solution was detected with colloidal gold test strip, and detection time was only 3-5 minutes. Detection limits of 15 quinolones were in the range of 0.3-5 μg·kg^-1. 400 batches of the samples were detected, and 9 batches of positive samples were found. The result was verifyied by UHPLC-MS/MS.