Abstract: The shredded filter membrane was extracted ultrasonically for 60 min with acetonitrile at 35℃. The extract was centrifugated for 10 min at the speed of 12 000 r·min^-1. HPLC was applied to the determinaton of 16 polycyclic aromatic hydrocarbons in the supernatant. Agilent Eclipse PAH-C₁₈ chromatographic column was used as separation column, and the mixture of acetonitrile and water with different mixing ratios was used as mobile phase in the gradient elution. Photodiode array detector and fluorescence detector were adopted in the determination. Linear relationships between values of peak area and mass concentration of the 16 polycyclic aromatic hydrocarbons were kept in the same range of 0.02-1.00 mg·L^-1, with detection limits (3S/N) in the range of 0.05-0.25 ng·m^-3. On the base of blank filter membrane, test for recovery was made by standard addition method; values of recovery found were in the range of 87.8%-103%, with RSDs (n=6) of recovery amounts less than 5.0%.