Abstract:
A portion of the sample (0.100 0 g) was dissolved in water and diluted to an appropriate volume according to the concentration of the analyte in sample. The ion-exchange column PRP-X100 was chosen as separation column, which was proved to have high efficiency in separation of chloroplatinic acid and carboplatin, due to bonding of polymer of divinylbenzene/triethylamine on the stationary phase. 60 mmol·L
-1 NH
4NO
3 solution (pre-adjusted its acidity to pH 5.5 with dil. NH
3·H
2O or dil. HNO
3) was used as mobile phase in the gradient elution program. Analyte in respective eluate was then determined by ICP-MS according to the prescribed procedure. As shown by the results, linearity ranges for chloroplatinic acid and carboplatin were found between 1.00 and 100
μg·L
-1 and between 500.0 and 1 000
μg·L
-1 with detection limits (3S/N) of 0.1
μg·L
-1 and 0.01
μg·L
-1respectively. Testes for recovery were performed by standard addition method using a substantial sample as matrix, giving values of recovery of 90.0%-98.8% for chloroplatinic acid and of 98.5%-99.2% for carboplatin. Values of RSDs (
n=6) found was 2.7%-3.4% for chloroplatinic acid and 2.1%-2.8% for carbonplatin. It was found that in the analysis of a substantial sample by the proposed method, as the concentration of carboplatin found was 1 mg·L
-1, H
2PtCl
6 was not inspected in the same sample. Hence, inspection of traces of platinum(Ⅳ) was attained directly in the sample solution without any enrichment manipulation.