基于光谱净信号算法的紫外分光光度法快速同时测定复方盐酸阿米洛利片中的盐酸阿米洛利和氢氯噻嗪

Fast and Simultaneous Determinations of Amiloride Hydrochloride and Hydrochlorothiazide in Compound Amiloride Hydrochlorothiazide Tablets by UV Spectrophotometry with Using Spectral Net Signal Algorithm

摘要: 结合光谱净信号算法，对紫外光谱相互干扰的复方盐酸阿米洛利片中盐酸阿米洛利和氢氯噻嗪进行快速同时测定。在240~400 nm内，通过光谱净信号算法计算盐酸阿米洛利和氢氯噻嗪的光谱净信号，两种组分的质量浓度与其光谱净信号分别在1.6~2.4 mg·L^-1和16~24 mg·L^-1内呈线性关系。盐酸阿米洛利和氢氯噻嗪的平均回收率分别为105%~106%和104%~105%。测定结果的相对标准偏差（n=5）分别为0.77%，0.69%。

Abstract: The mutual interference of the UV-spectra of the two effective components amiloride hydrochloride and hydrochlorothiazide contained in the compound amiloride hydrochlorothiazide tablets was solved by the application of the spectral net signal algorithm, and fast and simultaneous determination of the two effective components was performed. In the UV-spectral range of 240-400 nm, the net spectral signals of amilolide hydrochloride and hydrochlorothiazide were calculated by the spectral net signal algorithm, and linear relationships between net spectral signals and mass concentration of the two components were found in ranges of 1.6-2.4 mg·L^-1 and 16-24 mg·L^-1, respectively. Average recoveries of the two components were found in ranges of 105%-106% and 104%-105%, with RSD (n=5) of 0.70% and 0.69%, respectively.