Abstract: Determination of related impurities in ebastine tablets by high performance liquid chromatography. Phenyl bonded silica gel xtimate phenylhexyl chromatographic column (4.6 mm×250 mm, 5 μm) was used for separation, with phosphate buffer solution-acetonitrile (60+40) mixed solution was used as the mobile phase A, and the phosphate buffer solution-acetonitrile (70+30) mixed solution was used as the mobile phase B at the flow rate of 1.0 mL·min^-1. As shown by the results, linear relationships between values of peak area and mass concentration of the 4 impurities were found in the definite ranges, with detection limits (3S/N) in the range of 0.16-0.33 mg·L^-1. Test for recovery by standard addition method was made on the base of ebastine tablet sample, giving results in the range of 96.0%-104%, and RSDs (n=6) ranged from 0.83% to 4.2%.