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    银纳米粒子结合智能手机测定牛奶中链霉素

    Determination of Streptomycin in Milk Based on Silver Nanoparticles with Smartphone

    • 摘要: 利用槲皮素和硝酸银合成了槲皮素包覆的银纳米粒子,合成的银纳米粒子在测定链霉素时其溶液颜色由黄色变为樱桃红色,使用智能手机的图像处理软件获得红(R)绿(G)蓝(B)数据计算G/B的值,从而实现了牛奶中链霉素的测定。链霉素的浓度在10.00~150.00 μmol·L-1内与其对应的G/B值呈线性关系,检出限(3s/k)为0.63 μmol·L-1,测定下限(10s/k)为2.08 μmol·L-1。在30.00,70.00,130.00 μmol·L-1等3个浓度水平进行加标回收试验,回收率为97.9%~102%,测定值的相对标准偏差(n=6)为0.90%~2.8%。

       

      Abstract: Quercetin-coated silver nanoparticles were synthesized by quercetin and AgNO3. When using synthesized silver nanoparticles to determine streptomycin, the color of the solution changed from yellow to cherry red. Determination of streptomycin in milk could be realized by using smartphone which was used to obtain R(red)G(green)B(blue) values to calculate G/B values through image processing software. Linear relationship between G/B values and concentration of streptomycin was obtained in the range of 10.00-150.00 μmol·L-1, with detection limit (3s/k) of 0.63 μmol·L-1 and lower limit of determination (10s/k) of 2.08 μmol·L-1. Tests for recovery were made by standard addition method at the concentration levels of 30.00, 70.00, 130.00 μmol·L-1, giving values of recovery and RSDs (n=6) in the ranges of 97.9%-102% and 0.90%-2.8% respectively.

      WT5HZ

       

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