Abstract: The surface water sample was collected in a brown glass bottle, and then filtered on 0.2 μm polyethersulfone needle filter. UHPLC-MS/MS was applied to the rapid determination of 5 pollutants, i.e. aniline,benzidine, microcystin-LR, carbaryl and acrylamide in the filtrate. ACQUITY UPLC®BEH C₈ chromatographic column was used as stationary phase, and the mixture of methanol and 0.1% (volume fraction) formic acid solution mixed in different ratios was used as mobile phase in gradient elution. ESI⁺ and multi-reactions monitor mode were adopted in MS/MS. Linear relationships between values of intensity of mass spectral response and mass concentration of the 5 pollutants were kept in definite ranges, with detection limits in the range of 0.04-0.10 μg·L^-1. On the base of blank surface water sample, test for recovery was made by standard addition method; values of recovery found were in the range of 95.0%-120%, with RSDs (n=6) of determined values in the range of 1.9%-12%.