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    抑制褪色光度法测定啤酒中微量二氧化硫

    Determination of Trace Sulfur Dioxide in Beer by Using Inhibition Fading Spectrophotometry

    • 摘要: 基于在硫酸介质中二氧化硫对溴酸钾氧化亚甲基蓝(MB)褪色具有抑制作用,建立了抑制褪色光度法快速测定啤酒中微量二氧化硫的方法。在25 mL比色管中依次加入1 mol·L-1硫酸溶液、2×10-4mol·L-1 MB溶液、0.1 mol·L-1溴酸钾溶液和经氢氧化钠和硫酸处理好的样品溶液,于50℃水浴中加热3.0 min,在660 nm处测量该体系的吸光度A及空白样品体系的吸光度A0,计算吸光度差值ΔA(ΔA=A-A0)。结果显示,二氧化硫的质量浓度在0.04~0.4 mg·L-1内与ΔA呈线性关系,检出限(3s/k)为0.001 mg·L-1。对实际样品进行加标回收试验,回收率为96.5%~101%,测定值的相对标准偏差(n=11)为1.8%~2.8%。用该方法分析熟啤和生啤,将得到的测定值与国家标准方法GB 5009.34-2016的进行了比对,相对误差为-1.5%~2.0%。

       

      Abstract: Based on the principle that sulfur dioxide can inhibit the fading of MB oxidized by potassium bromate in sulfuric acid medium, a method for rapid determination of trace sulfur dioxide in beer by inhibiting fading spectrophotometry was established. 1 mol·L-1 sulfuric acid solution, 2×10-4mol·L-1 MB solution, 0.1 mol·L-1 potassium bromate solution and sample solution treated with sodium hydroxide and sulfuric acid were added sequentially to the 25 mL colorimetric tube, the mixed solution was heated in a water bath at 50℃ for 3.0 min. The absorbance of the system (A) and the blank sample system (A0) were measured at 660 nm, to obtain the difference in absorbance (ΔA, ΔA=A-A0). The results showed that the mass concentration of sulfur dioxide had a linearity relationship with ΔA in the range of 0.04-0.4 mg·L-1, with detection limit (3s/k) of 0.001 mg·L-1. The recovery test was made on the actual sample by the standard addition method, giving recoveries ranged from 96.5% to 101%, and RSDs (n=11) of determined values were found in the range of 1.8%-2.8%. The proposed method was used in the analysis of cooked beer and draft beer, and determined values obtained were compared with those obtained by national standard method of GB 5009.34-2016, with relative error in the range of -1.5%-2.0%.

       

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