Abstract: HPLC combined with ultrasonic extraction was applied to the simultaneous determination of five isomeric CMRs (including BjF, BeP, BbF, BkF, and BaP) in textiles. The sample (0.500 g) was extracted by ultrasonic with 20 mL of the mixed solvent of methanol and tetrahydrofuran with volume ratio of 1:1 for 30 min at 30℃. The extract was separated on an Agilent Eclipse PAH chromatographic column with the mixture of acetonitrile and water as the mobile phase in gradient elution, and fluorescence detector was used for detection. As shown by the results, linearity relationships between values of peak area and mass concentration of five isomeric CMRs were found in the same range of 5.0-150 μg·L^-1, and detection limits (3S/N) were all 1.0 μg·L^-1. Recoveries obtained by standard addition method were in the range of 98.6%-103% and RSDs (n=6) of measured values were in the range of 2.9%-3.7%.