Abstract: Automatic solid phase extraction-liquid chromatography-tandem mass spectrometry (automatic-SPE-LC-MS/MS) was applied to rapid determination of the 7 antiviral drugs (induding amantadine, rimantadine, memantine, acyclovir, moroxydine, imiquimod and oseltamivir) residues in chicken. The sample was extracted with a mixture of acetonitrile-1% (mass fraction) trichloroacetic acid solution at volume ratio of 1:1, and purified by automatic-SPE system. The chromatographic separation was performed by using Hilic Plus hydrophilic chromatographic column as stationary phase, and the mixtures of methanol and 0.1% (φ) formic acid solution at different volume ratios as mobile phase for gradient elution. Isotope internal standard method was used for quantitative analysis. It was showed that linear relationships between peak area ratio of quantitative ion to internal standard and mass concentration of the 7 antiviral drugs were found in the same range of 1.0-20 μg·L^-1, with detection limits (3S/N) in the range of 0.03-0.3 μg·kg^-1. Test for recovery was made by standard addition method, giving results in the range of 75.7%-105%, and RSDs (n=6) of the determined values were less than 5.0%. Compared with national standard and industry standard, this method achieved automation of instrument on the premise of high extraction efficiency, and protected the experimental personnel from being exposed to organic reagents.