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    表面增强拉曼光谱法测定牛奶中青霉素G钠的残留量

    Determination of Penicillin G Sodium Residue in Milk by Surface Enhanced Raman Spectrometry

      摘要
    • 摘要: 青霉素G钠（NaBP）溶液的常规拉曼光谱信号微弱。将制备的浓缩银纳米粒子（AgNPs）20 μL与不同浓度（1×10-9~1×10-1mol·L-1）的青霉素G钠溶液（pH 6）20 μL混合，所得混合液的表面增强拉曼光谱（SERS）信号显著增强，在上述混合液中加入1×10-2mol·L-1硫酸镁溶液8 μL，青霉素G钠SERS增强效果最佳。据此，提出了以AgNPs为基底，硫酸镁为凝聚剂，采用SERS测定青霉素G钠含量的方法，并用于加标牛奶样品的检测。结果表明，青霉素G钠浓度为1×10-8~1×10-3mol·L-1时，其浓度的对数值与相应的SERS信号强度呈线性关系，检出限（3s/k）为8.2×10-9mol·L-1。按标准加入法进行回收试验，回收率为80.0%~96.0%，测定值的相对标准偏差（n=5）为2.3%~6.5%。

       

      Abstract: The normal Raman spectrum signal of penicillin G sodium (NaBP) solution was weak. 20 μL of concentrated silver nanoparticles (AgNPs) prepared were mixed with NaBP solution in different concentrations (1×10-9-1×10-1mol·L-1), and surface enhanced Raman spectrometry (SERS) signal of the mixture obtained was significantly enhanced. 8 μL of 1×10-2mol·L-1 magnesium sulfate solution was added into the above mixture, and the SERS enhancement effect of NaBP was the best. Therefore, a method for determination of NaBP by SERS with AgNPs as substrate and magnesium sulfate as coagulant was proposed, and this method was applied for determination of NaBP residue in milk. As shown by the results, linear relationship between the logarithm of NaBP concentration and the corresponding SERS signal intensity was found in the range of 1×10-8-1×10-3mol·L-1, with detection limit (3s/k) of 8.2×10-9mol·L-1. Test for recovery was made by standard addition method, giving results in the range of 80.0%-96.0%, with RSDs (n=5) of the determined values in the range of 2.3%-6.5%.

       

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