Abstract: A method for the determination of aluminum in alanyl glutamine injection by inductively coupled plasma mass spectrometry (ICP-MS) was proposed. 6 test samples (7.5 mL) were placed in 6 plastic centrifuge tubes of 15 mL, and a certain amount of aluminum standard solutions was added. Then the test spiked solution series were diluted by 5% (φ) nitric acid solution, and filtered by 0.22 μm water membrane. Standard addition method was used for quantitative analysis. Atomized gas to dilute high matrix sample introduction helium collision (HMI-He) mode was used for ICP-MS analysis. As shown by the results, linear relationship between value of mass concentration of aluminum in a test spiked solutions and its response value was kept within 30.00 μg·L^-1, with detection limit (3s) of 0.88 μg·L^-1. Test spiked solutions were determined for precision, and RSDs (n=6) of the determined values were less than 2.0%. Recovery test was made on the same test sample at 3 concentration levels by standard addition method, giving results in the range of 101%-106%. This method has been applied to the analysis of 12 batches of alanyl glutamine injections, and detection amounts of aluminum were in the range of 20.00-31.22 μg·L^-1.