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    顶空-气相色谱-质谱法分析线叶旋覆花不同入药部位中挥发性成分的差异

    Analysis of Difference of Volatile Components in Different Medicine Parts of Inula Lineariifolia by HS-GC-MS

    • 摘要: 将线叶旋覆花两个入药部位分开裁剪,用水洗净后干燥、粉碎,过孔径为0.25 mm筛网。分取0.02 g粗粉于20 mL顶空瓶中,于120℃加热30 min。所得挥发性成分进入气相色谱仪,以DB-5色谱柱在程序升温条件下分离,用配电子轰击离子源的质谱仪检测,以保留时间、碎片离子峰定性,以面积归一化法定量。结果显示:从线叶旋覆花中鉴定出的挥发性成分分别有46种(茎叶中)和30种(花中),其峰面积占总挥发性成分峰面积的71.11%,99.98%。花和茎叶中共有挥发性成分有8种,特有挥发性成分有22,38种;茎叶中挥发性成分包括烯烃类、酸类、醇类、醛类、烷烃类、芳香烃类、酯类、酮类和其他类化合物各13,5,4,9,7,2,3,2,1种,相对含量分别为18.11%,17.32%,12.16%,8.76%,4.05%,0.41%,0.80%,0.32%,9.18%;花中挥发性成分包括烯烃类、醇类、醛类、芳香烃类和其他类化合物各18,2,5,2,3种,相对含量分别为52.67%,29.35%,11.50%,2.07%,4.39%。不同入药部位中活性成分烯烃类化合物相对含量均较高,挥发性成分的种类和相对含量相差较大,但是相对含量大于2.00%的桉叶油醇、巴伦西亚橘烯、(-)-香树烯、γ-橄槛烯均有抗炎或抗肿瘤等药理作用,说明这两个入药部位均有较高的应用价值。

       

      Abstract: The two medicine parts of Inula lineariifolia (IL) were cut separately, washed with water, dried, and then crushed. The crude powder was passed through 0.25 mm sieve, an aliquot (0.02 g) was placed into 20 mL-headspace flask and heated at 120℃ for 30 min. The volatile components obtained were entered into gas chromatograph, separated on DB-5 chromatographic column under programmed temperature conditions, and detected by mass spectrometer with an electron impact ion source. Retention time and fragmentation ion peaks were applied to qualitative analysis, and the peak area normalization was adopted for quantitative analysis. It was shown that 46 (for stem and leaf), 30 (for flower) volatile components were identified from different medicine parts of IL, which peak area accounted for 71.11% and 99.98% of that of the total volatile components, respectively. Different medicine parts of IL had 8 common volatile components and 22, 38 unique volatile components, respectively. The volatile components of the stem and leaf consisted 13 olefins, 5 acids, 4 alcohols, 9 aldehydes, 7 alkanes, 2 aromatic hydrocarbons, 3 esters, 2 ketones and 1 others, which relative content (%, the same below) was 18.11%, 17.32%, 12.16%, 8.76%, 4.05%, 0.41%, 0.80%, 0.32%, 9.18%, respectively. The volatile components of the flower consisted 18 lefins, 2 alcohols, 5 aldehydes, 2 aromatic hydrocarbons and 3 others, which relative content was 52.67%, 29.35%, 11.50%, 2.07%, 4.39%. The relative content of the active ingredient olefins was comparatively high in different medicine parts, and the variety and relative content differed considerably. However, eucalyptol, valencene, (-)-alloaromadendrene and γ-maalinenee, which relative content was greater than 2.00%, had pharmacological properties such as anti-inflammatory or anti-tumor effects, indicating that the two medicine parts of IL were of high value for application.

       

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