Abstract: Electrochemical DNA sensor was a sensor constructed based on base complementary pairing rules between DNA probe and target DNA, which detected targets according to the change of signal before and after the binding of recognition elements and targets, and had become an effective alternative to traditional detection methods. Metal-organic framework materials (MOFs) had many advantages, including large specific surface area, high porosity, adjustable pore size and high thermal stability, attracting wide attention from scholars. MOFs had been initially used for the construction of electrochemical DNA sensors, which were applied to the sensitive and accurate detection of tumor markers, antibiotics and heavy metals. Therefore, the DNA probe immobilization methods and signal substances of electrochemical DNA sensors were reviewed, the application progress of electrochemical DNA sensors based on MOFs in the field of analysis and detection were focused, and its future development direction was prospected (50 ref. cited).