Abstract: Aptamer exhibits unique technical advantage in the aspects of specific recognition on target and signal transduction. Combing aptamer and molecular imprinted technology, the obtained aptamer functionalized molecular imprinted polymer exhibits excellent capability of selective recognition and sensing performance. Based on this, the preparation methods of aptamer functionalized molecularly imprinted polymer were introduced, and their applications in biosensor, separation and enrichment were reviewed and prospected (73 ref. cited).