Abstract: A method for determination of 9 biogenic amines including tryptamine, phenylethylamine, putrescine, cadaverine, histamine, octopamine, tyramine, spermidine and spermine in water body by high performance liquid chromatography combined with solid phase extraction and pre-column derivatization was proposed. 10 mL of water sample was taken and placed in a 15 mL-centrifuge tube. 250 μL of 100 mg·L^-1 mixed internal standard solution and 3 mL of 50 mmol·L^-1 hydrochloric acid solution were added. The mixture was mixed and passed through a solid-phase extraction column (weak cation exchange column) at a flow rate of 3 mL·min^-1. 2 mL of water, 2 mL of methanol and 4 mL of methanol solution containing 2 g·L^-1 sodium hydroxide were added in sequence. The eluent was collected and placed in a 15 mL-centrifuge tube (pre-added with 250 μL of 1 mol·L^-1 hydrochloric acid solution), and blown to near drynesss by nitrogen. 300 μL of saturated sodium bicarbonate solution was added, and the solution was made its volume up to 1 mL with water. 1 mL of buffer solution (pH 10.5) was added and the mixture was mixed well. 1 mL of 10 g·L^-1 dansyl chloride derivative solution was added, and the solution was vortexed and heated at 60 ℃ for 15 min. The solution was cooled to 25 ℃, and 200 μL of ammonia was added. The solution was heated at 60 ℃ for 10 min and cooled to 25 ℃. 0.5 g of sodium chloride and 0.5 mL of acetonitrile were added. The mixture was mixed well and centrifuged for 1 min. The supernatant was passed through a 0.22 μm filter membrane, and 9 biogenic amines in the filtrate was determined using Waters XBridge C₁₈ chromatographic column as the stationary phase and the mixed solution composed of 0.01 mol·L^-1 ammonium acetate solution containing 0.1% (volume fraction) acetic acid and acetonitrile at different volume ratios as mobile phase for gradient elution, with the internal standard method for quantification. As shown by the results, linear relationships between the ratios of the corresponding derivative peak area to the internal standard peak area and both mass concentration ratios of 9 biogenic amines were found in the range of 1.0-100.0 mg·L^-1, with detection limits (3.143s) in the range of 0.03-0.08 mg·L^-1. Test for recovery was made by the standard addition method, giving results in the range of 76.4%-107%, with RSDs (n=6) of the determined values not more than 5.0%. This method was used for the analysis of 18 actual water samples, and phenylethylamine was commonly detected, with the detection amounts in the range of 0.03-0.13 mg·L^-1.