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    基于核磁指纹图谱和化学计量学方法的川麦冬质量评价

    Quality Evaluation of Sichuan Ophiopogon Japonicus Based on Nuclear Magnetic Fingerprints and Chemometrics Methods

    • 摘要: 通过对比绵阳市、达州市、广安市3个产区川麦冬的核磁指纹图谱,采用相似度分析、聚类分析、主成分分析(PCA)和偏最小二乘法判别分析(PLS-DA)4种化学计量学方法分析各样品核磁指纹图谱特点并进行产区鉴别,还对总黄酮和总皂苷进行核磁积分相对定量比较。结果表明,不同产区的川麦冬样品存在明显差异,同一大产区中的不同小产区的样品间也存在一定差异。相似度分析、聚类分析、PCA和PLS-DA结果具有一致性,说明核磁指纹图谱结合化学计量学方法可用于产区鉴别。相对定量结果显示绵阳产区麦冬标志化合物总皂苷和总黄酮含量显著高于其他产区(P<0.05)。

       

      Abstract: By comparing the nuclear magnetic fingerprint of Sichuan Ophiopogon japonicus in Mianyang, Dazhou, and Guang’an regions, four chemometrics methods, including similarity analysis, cluster analysis, principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA), were used to analyze the characteristics of the nuclear magnetic fingerprints of samples and identify the regions. The total flavonoids and total saponins were compared quantitatively by nuclear magnetic integration. As shown by the results, there were significant differences in the samples of Sichuan Ophiopogon japonicus from different regions, and there were also certain differences between samples from different small regions within the same large region. The consistency of the results of similarity analysis, cluster analysis, PCA, and PLS-DA indicated that the combination of nuclear magnetic fingerprints and chemometrics methods could be used for identifying regions. It was shown by the relative quantitative results that the total saponin and total flavonoid contents of the marker compounds of Ophiopogon japonicus in the Mianyang region were significantly higher than those in other regions (P<0.05).

       

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