Abstract: To realise the identification and quality control of parts of Epimedium brevicornuin Maxim., the infrared spectra difference of different parts of wild Epimedium brevicornuin Maxim. from northern and southern slopes of Funiu Mountain was analyzed and compared. The collected wild Epimedium brevicornuin Maxim. samples were divided into 4 parts of roots, fibrous roots, stems, and leaves. After drying in the shade, crushing, and sieving, the raw infrared spectra were obtained by Fourier transform infrared spectrometer. After smoothing noise reduction, baseline correction, normalization, and derivative, the second-order derivative infrared spectra were obtained. The differences in infrared spectra between the 4 parts of wild Epimedium brevicornuin Maxim. from northern and southern slopes were compared, and the reason of the differences was analyzed in combination with the characteristics of the microclimate. It was shown that there were differences in raw infrared spectra and second-order derivative infrared spectra of the 4 parts of wild Epimedium brevicornuin Maxim. from Funiu Mountain. For second-order derivative infrared spectra, the roots and fibrous roots of Epimedium brevicornuin Maxim. had double peaks at 1 466, 1 452 cm⁻¹ and 1 466, 1 453 cm⁻¹, and single peaks at 938 cm⁻¹ and 937 cm⁻¹, which could be differentiated from stems and leaves. Fibrous roots had a strong and sharp absorption peak at 1 260 cm⁻¹, which could be differentiated from roots. Stem had a strong and sharp peak at 1 648 cm⁻¹, which could be differentiated from the other parts. Leaves had an obvious absorption peak at 1 440 cm⁻¹, which could be differentiated from the other parts. The chemical components in Epimedium brevicornuin Maxim. from northern and southern slopes of mountains were similar as a whole, but there were also differences. Epimedium brevicornuin Maxim. from northern slopes of mountain accumulated more substances than that from southern slopes of mountain, especially in roots and stems,which might be related to the difference in illumination duration and illumination intensity of the 2 regions.