高级检索

    栀子柏皮汤复方中药高效液相色谱指纹图谱的建立

    Establishment of High Performance Liquid Chromatography Fingerprint of Zhizi Baipi Decoction Compound Traditional Chinese Medicine

    • 摘要: 按照《伤寒论》收载的方法制备10批栀子柏皮汤供试品溶液,在Diamonsil C18色谱柱上进行分离,在柱温35 ℃条件下以不同体积比的0.2%(体积分数)甲酸溶液-甲醇混合溶液为流动相进行梯度洗脱,在波长250 nm处检测,记录色谱图。根据中药色谱指纹图谱相似度评价系统(2012版)建立了栀子柏皮汤复方中药的高效液相色谱(HPLC)指纹图谱。结果显示:10批栀子柏皮汤指纹图谱中有15个共有峰,其中3个共有峰归属于黄柏,其余共有峰均归属于栀子。与对照品比对,确认9、10、13号峰分别为京尼平龙胆双糖苷、栀子苷、盐酸小檗碱。10批栀子柏皮汤指纹图谱与对照指纹图谱的相似度为0.880~0.949。

       

      Abstract: According to the method recorded on Shanghan Lun, the test solutions of 10 batches of Zhizi Baipi decoction were prepared and separated on Diamonsil C18 column, using the mixed solution composed of 0.2% (volume fraction) formic acid solution and methanol at different volume ratios as mobile phase for gradient elution at 35 ℃ of column temperature. The components of the test solutions were detected at 250 nm of wavelength, and the chromatogram was recorded. High performance liquid chromatography(HPLC) fingerprint of Zhizi Baipi decoction compound traditional Chinese medicine was established according to the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (2012 Aedition). As shown by the results, there were 15 common peaks in the fingerprints of 10 batches of Zhizi Baipi decoction, in which 3 common peaks were attributed to Phellodendron amurense, while the remaining common peaks were attributed to Gardenia jasminoides. Compared with the reference substance, peaks of No.9, No.10 and No.13 were genipin 1-gentiobioside, geniposide, and berberine hydrochloride, respectively, and the similarity was in the range of 0.880-0.949 between fingerprints of 10 batches of Zhizi Baipi decoction and reference fingerprint.

       

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