Abstract: After adding the mixed solution of methanol and acetonitrile at volume ratio of 8∶2 into samples of medical dressing category medical device products, vortex mixing, ultrasonic extraction, and centrifugal separation were performed. The supernatant was passed through a 0.22 μm filter membrane, and the filtrate was introduced into the high performance liquid chromatograph. Hydroxybenzoate compounds (PBs) were separated by gradient elution with mixed solutions of acetonitrile and 0.12% (volume fraction) phosphoric acid solution at different volume ratios on the ShimNex CS C₁₈ column, and quantified by the external standard method. It was shown that linear relationships between values of mass concentration and peak area of 17 PBs were kept in the range of 1-50 mg·L⁻¹, with detection limits (3S/N) in the range of 5-18 mg·kg⁻¹. Test for recovery was made according to the standard addition method, giving recoveries in the range of 97.6%-108%, and RSDs (n=6) of the determined values ranged from 0.30% to 4.4%. This method was used to screen 10 batches of actual samples with different matrices, and the detection rate of PBs was 40%. Methyl p-hydroxybenzoate was detected in 1 batch of samples, and ethyl p-hydroxybenzoate and propyl p-hydroxybenzoate were detected in 3 batches of samples.