Abstract: Given that microbial fermentation has become the mainstream method for producing 1-deoxynojirimycin （1-DNJ）, the matrix of the fermentation broth is relatively complex, and the detection of 1-DNJ is difficult with no relevant reports available. Therefore, the study mentioned by the title was conducted. The 0.100 0 g of fermentation broth sample of bacteria extracted from yak milk was taken, its volume was made up to 10 mL with water, and the mixture was purified through a 0.22 μm filter membrane and an activated RP pre-processing cartridge after ultrasonic dissolution for 5 min. The first 3 mL of eluate was discarded, and 2 mL of the subsequent eluate was collected for ion chromatography analysis. Using a Dionex CarboPac^TM MA1 anion analytical column at column temperature of 30 ℃ and flow rate of 0.30 mL·min⁻¹, gradient elution was performed with the mobile phase consisting of 1 000 mmol·L⁻¹ sodium hydroxide solution and water. Quantification of 1-DNJ was carried out using a quadruple-potential waveform pulsed amperometric detector. It was shown that linear relationship between the mass concentrations of 1-DNJ and corresponding peak areas was kept in the range of 0.01–1.64 mg·L⁻¹, with detection limit （3S/N） of 0.63 μg·L⁻¹. Tests for precision and spike recovery were performed on actual samples, showing RSDs （n=6） of the determined values of 1.8%, and recoveries in the range of 98.8%–101%. The proposed method was applied to the analysis of 5 batches of actual samples, and the highest detected amount of 1-DNJ was 1.34 μg·g⁻¹.