高效液相色谱法测定虎杖中白藜芦醇、白藜芦醇苷和游离大黄素

Simultaneous HPLC Determination of Resveratrol,Polydatin and Free Emodin in Polygonum Cuspidatum

摘要: 用高效液相色谱法同时测定虎杖中白藜芦醇、白藜芦醇苷和游离大黄素含量.色谱柱为Waters Symmetry C18柱(3.9 mm×150 mm,5 μｍ),流动相为0.015 mol·L-1 H3PO4-甲醇,流速为0.5 mL·min-1,检测波长为284 nm.样品采用乙醇-水(1+1)溶液和乙酸乙酯提取.江西产虎杖样品中白藜芦醇、白藜芦醇苷和游离大黄素的回收率分别为97.9%,96.1%,95.6%,相对标准偏差小于5%.

Abstract: HPLC with gradient elution,was applied to the simultaneous determination of resveratrol,polydation and free emodin in polygonum cuspidatum. Waters symmetry C18 column (3.9 mm×150 mm,5 μm) was used as chromatographic column. Mobile phase used was a mixed solution containing methanol and dil. phosphoric acid cH3PO4: 0.015 mol·L-1 and a flow-rate of 0.5 mL·min-1 was used in the elution. UV-detection at 284 nm was used in the determination. Pre-treatment of sample was performed by ultrasonic extraction with ethanol-H2O (1+1) and followed by 2 successive extraction with ethylacetate. Recovery was tested for resveratrol,polydatin and free emodin in polygonum cuspidatum grown in Jiangxi provicince,and the results obtained were 97.9%,96.1% and 95.6% respectively,with RSD′s less than 5%.