Abstract: Amounts of 3 anti-dandruff agents in shampoo cosmetics,including salicylic acid,ketoclonazole and climbazole,were simultaneously determined by UPLC.ACQUITY UPLC-BEH C18 column (2.1 mm×100 mm,1.7 μm) was used as stationary phase,and mixture contaning acetonitrile methanol and phosphate-EDTA buffer solution of pH 4 (57.5+8.5+34) was used as mobile phase in the UPLC with DAD at the wavelength of 230 nm.Linear relationships between values of peak areas and mass concentrations of 3 anti-dandruff agents were obtained,with linear ranges of 50-500 mg·L-1 (for salicylic acid),50-500 mg·L-1 (for ketoclonazole),and 100-1 000 mg·L-1 (for climbazole).The proposed method was used in the determination of 3 anti-dandruff agents in shampoo cosmetics,values of recovery found by standard addition method were in the range of 94.2%-99.4%,with values of RSD′s (n=6) less than 0.35%.