高效液相色谱法测定日化用品中苏丹红类着色剂
HPLC Determination of Sudan Red Colorants in Daily-Use Chemical Articles
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摘要: 应用高效液相色谱法测定了日化用品中苏丹红类着色剂(包括苏丹红Ⅰ、Ⅱ、Ⅲ及Ⅳ)的含量。样品用丙酮-乙腈(1+3)混合溶液提取后,经ZORBAX Eclipse XDB-C8色谱柱分离,以不同体积比的甲酸-水(0.1+99.9)溶液和甲酸-乙腈(0.1+99.9)溶液为流动相梯度洗脱,用二极管阵列检测器检测。苏丹红Ⅰ的检测波长为478 nm,苏丹红Ⅱ、Ⅲ的检测波长为500 nm,苏丹红Ⅳ的检测波长为520 nm。4种苏丹红的线性范围均为0.50~50 mg·L-1,检出限(3S/N)均为0.05 mg·L-1。以空白样品为基体,进行加标回收试验,苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ的回收率在92.0%~102.1%之间。Abstract: HPLC was applied to the determination Sudan red colorants including Sudan Ⅰ, Ⅱ, Ⅲ and Ⅳ in daily chemical articles. The sample was extracted with acetone-acetonitrile (1+3) mixture. The extract was separated on ZORBAX Eclipse XDB-C8 column by gradient elution with eluants containing different proportions of solution of A ［HCOOH-H2O (0.1+99.9)］ and B ［HCOOH-CH3CN(0.1+99.9)］, and determined with diode array detector at the wavelengths of 478 nm for Sudan Ⅰ, 500 nm for Sudan Ⅱ and Ⅲ, 520 nm for Sudan Ⅳ. Linearity ranges for the 4 Sudan red colorants were found same in the range of 0.50-50 mg· L-1, with detection limit (3S/N) of 0.05 mg· L-1. Using blank sample as matrix, recovery test was made by standard addition method, values of recovery found were in the range of 92.0%-102.1%.
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