胶束扫集-电动色谱分离-毛细管电泳法测定升麻中三种有机酸含量
Capillary Electrophoretic Determination of 3 Organic Acids in Rhizome Cimicifugas Foetidae with Enrichment by Micellar Sweeping and Separation by Electrokinetic Chromatography
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摘要: 采用电场增强胶束扫集-电动色谱-毛细管电泳法对升麻中异阿魏酸、阿魏酸和咖啡酸的含量进行了测定,电泳缓冲体系为90 mmol·L-1 SDS-20 mmol·L-1 NaH2PO4(pH 2.20)-甲醇(10+90),分离电压为20 kV,检测波长214 nm.在优化的试验条件下,胶束扫集-电动色谱法对异阿魏酸、阿魏酸和咖啡酸富集倍数为4.3,4.8,2.9,3种有机酸均在14 min内出峰,线性范围分别为0.50~20.0,0.50~20.0,1.0~40.0 mg·L-1.应用此方法分析了升麻样品并进行了回收率和精密度试验,测得回收率在93.2%~113.3%之间,测定值的相对标准偏差(n=5)小于6%.Abstract: Micellar sweeping-electrokinetic chromatography-capillary electrophoresis was applied to the determination of iso-ferulic acid,ferulic acid and caffeic acid in rhizome cimicifuagae foetidae under enhanced electric field intensity.A mixed solution containing 90 mmol·L-1 SDS,20 mmol·L-1 NaH2PO4 (pH 2.20) and methanol (amounting to 10% by volume of the solution) was used as the electrophoretic medium.Separation voltage applied was 20 kV and wavelength of detection was taken at 214 nm.Under the optimum conditions,values of multiple of enrichment of 4.3,4.8 and 2.9 were attained for iso-ferulic acid,ferulic acid and caffeic acid respectively.The analysis of a sample was completed within 14 minutes.Linearity ranges of the 3 acids were kept in the ranges of 0.50-20.0,0.5-20.0 and 1.0-40.0 mg·L-1 respectively.Recovery and precision were tested and values of recovery and RSD′s (n=5) found were in the ranges of 93.2%-113.3% and <6% respectively.
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