Abstract: UHPLC was applied to the determination of 4 nitrofurans, i.e. furacilin, furaltadone, nitrofurantoin and furazolidone in water with solid phase extraction. The sample was purified by passing through HLB solid phase extraction column and eluted with a mixture of ammonia and methanol (5+95). BEH C18 chromatographic column was used as stationary phase, and a mixture of acetonitile and 2 mmol·L-1 ammonium acetate solution (containing 0.1% formic acid) (23+77) was used as mobile phase. UV-detection at 360 nm was adopted in the determination. Linear relationships between values of peak area and mass concentration of the 4 nitrofurans were kept in the same range of 5.0-200 μg·L-1, with detection limit (3S/N) of 0.02 μg·L-1.On the base of blank sample, test for recovery was made by standard addition method;values of recovery found were in the range of 84.5%-97.2%, with RSD′s (n=6) in the range of 1.5%-4.5%.