Abstract: Sample of sucker secretion of Boston ivy was extracted with a mixture of methanol and chloroform (2+1), and after centrifugation, the supernatant was taken for analysis. Fifteen volatile organic compounds with Mr less than 450, including the main components of sitosterol, methyl-α-D-glucopyranoside, 2,4-bis-(1,1-dimethylethyl)-phenol, stigmasterol and palmitic acid, were identified by GC-MS; and for 19 hardly volatile organic compounds with Mr between 220 and 920, their empirical formulae and Mr, were confirmed by HPLC/Q-TOFMS. It was found that 12 of the 19 compounds were carbohydrates containing both N and O, and 3 of them containing also S in addition to N and O. It was deduced that these compounds were easy to produce hydrogen bonds, and that the interaction of hydrogen bonds was one of the factors related to the climbing property of the Boston ivy.