Abstract: Volatile oil of Elsholtzia bodinieri vaniot grown in Ziwuling was extracted by the supercritical CO2 fluid and its components was separated and characterized by GC-MS.Forty three ingredients were found in the volatile oil and among them 41 compounds were identified under optimun analytical conditions.The main components were as follows: thymol,carvacrol,elshtzitol,6,10,14-trimethyl-2-pentadecanone,β-citronellol,elsholziaketone and etc.The components and their contents found in this volatile oil were found some what different from those reported in literature,and in addition,some new components were found for the first time.The method proposed in this paper showed its special features of good in accuracy and precision,stability and reliability,and was feasible to be used in the separation and determination of chemical components in volatile oil of Chinese herbal medicines.