Abstract: HPLC-MS/MS was applied to the rapid determination of residual amount of forchlorfenuron in fruits. The fruit sample was extracted with acetonitrile by homogeneous stirring and purified with adsorbents of N-propyl-ethylene diamine, C18 and anhydrous magnesium sulfate. The purified sample solution was separated by using C18 column as stationary phase, and mixtures of methanol and 5 mmol·L-1 ammonium acetate solution mixed in different ratios were used as mobile phase in gradient elution. ESI+ as well as multi-reaction monitoring was adopted in MS/MS. Linear relationship between values of peak area and mass concentration of forchlorfenuron was kept in the range of 0.005-0.200 mg·L-1, with lower limit of determination (10S/N) of 10 μg·kg-1. Tests for recovery were made at concentration levels of 0.01, 0.05 and 0.10 mg·L-1 of standard solution, values of recovery found were in the range of 86.4%-106% with RSD′s (n=6) in the range of 3.2%-9.5%.