Abstract: HPLC was applied to the simultaneous determination of rutin and quercetin in branches of Sophora aponica L.ZORBAX Eclipse XDB-C18 column (4.6 mm×250 mm,5 μm) was used as stationary phase,and a mixture of methanol and 0.01 mol·L-1 ammonium acetate solution (47+53) was used as mobile phase in the HPLC analysis with DAD detection at the wavelength of 360 nm.Linear relationships between values of peak area and mass of rutin and quercetin were kept in ranges of 0.015 4-0.154 0 mg·L-1 and 0.010 8-0.108 0 mg·L-1 with detection limits (3S/N) of 0.06,0.008 μg·g-1 respectively.Nine samples were analyzed for contents of rutin and quercetin by the proposed method,values of recovery found by standard addition method were in ranges of 97.4%-98.8% and 97.4%-98.2%,and values of RSD′s (n=6) found were 1.6% and 1.8%,respectively.