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    红树植物木榄叶的基本化学成分的分析

    Analysis of Leaves of Bruguiera Gymnorrhiza for Its Elementary Chemical Composition

    • 摘要: 对红树植物木榄叶的基本化学成分作了较全面的分析.对其水分、灰分及灰分中的酸不溶物和硫等组分的测定.直接从新鲜的树叶取样,采用120H型电子水分测定仪测定其水分,而灰分则将试样在高温炉中干法灰化后按国标GB 5009.4-1985中所述方法测定.试样中的粗蛋白及粗脂肪则分别按国标GB 5009.4-1985和GB 5009.3-1985中所述方法测定.供氨基酸及微量元素测定的树叶样品,需经在空气中凉干并粉碎.按需要取一定量的样品,用6 mol·L-1盐酸浸取和用活性碳脱色后所得溶液中分别用氨基酸自动分析仪和电感耦合等离子体原子发射光谱法进行测定.共测得17种氨基酸,其总量达14.0 mg·g-1,其中约占总量30%的7种氨基酸为人体所必需的.在树叶样品中还测定了15种微量无机元素,其中大部分属有营养价值的矿物质元素.

       

      Abstract: Sample of leaves of Bruguiera gymnorrhiza was analyzed for its elementary chemical composition.Fresh leaf sample was used for the determination of moisture,ash,acid-insoluble substance and sulfur in the ash and etc.Moisture was determined by the 120H electronic moisture determinator,and ash was determined as described in GB 5009.4-1985.Crude protein and fats were determined by the methods given in GB 5009.4-1985 and GB 5009.3-1985 respectively.Air-dried leaves were smashed and taken as sample for determination of micro amounts of inorganic elements and amino acids.Amino acids in the sample were extracted with 6 mol·L-1 HCl and decolored with active carbon.The filtrate obtained was used for determination of amino acid with the automatic amino acid determinator.ICP-AES method was applied to the determination of micro amounts of inorganic elements,with the sample treated in a similar way as in the determination of amino acids.Seventeen amino acids,amounting totally to 14.0 mg·g-1,were found in the leaf-sample,among which 7 amino acids,about 30% of the total,were essential for human health.Fifteen inorganic elements,i.e.B,Ba,Ca,Cu,Fe,K,Mg,Mn,Na,Ni,P,S,Si (soluble),Sr and Zn,were found,most of which were nutritious mineral elements.

       

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