Abstract: An optimized procedure for the extraction of onion pigment from onion was described.Antioxidation activity of the extracted onion pigment was studied by the Fenton reaction and the method of DPPH (1-diphenyl-2-picrylhydrazino) free radical (·DPPH).As indicated by the experimental results,onion pigment shown scavenging action on both the hydroxyl free radical (·OH) and 1-diphenyl-2-picrylhydrazino free radical (·DPPH) and gave their rates of scavenge of 42.4% and 58.2% respectively.The results of this work provided a sound scientific basis supporting the exploitation of the natural onion pigment for its application in both the food industry and preparation of nutriments.