Abstract: It was reported in this paper that curcumine was separated from curcuma and determined by capillary electrophoresis with high-frequency conductivity detection in non-aqueous medium within 12 min. Optimum conditions for the analysis was described as follows: ① separation medium: anhydrous methanol;② electrolyte: 6 mmol·L-1 HOAc+8 mmol·L-1 NH4OAc solutions (pH 4.5);③ separation voltage: 25.0 kV. Linear relationship was kept between peak area and concentration of curcumine in the range from 3.0 to 140.0 mg per L of solution (r=0.997),with a detection limit of 0.5 mg·L-1. Samples of curcuma grown in Sichuan,Guangdong and Guangxi were collected and analyzed by the proposed method. Values of recovery test were in the range from 95.3%-100.9%.